The Mad Hatter has escaped from prison, and is on a quest to add Batman's cowl to his collection of hats. He attends Gotham City's annual headdress ball disguised as the Three-tailed Pasha Of Panchagorum, and snatches a large ruby off of columnist Hattie Hatfield's headdress. The villain makes a getaway, but not before turning Batman's cowl pink with a radioactive spray. He trails Batman to the Atomic Energy Laboratory, knowing he will have a chance to snatch his cowl when it is removed for decontamination.
Wealthy entrepreneur Bruce Wayne and his ward Dick Grayson lead a double life: they are actually crime fighting duo Batman and Robin.
A secret Batpole in the Wayne mansion leads to the Batcave, where Police Commissioner Gordon often calls with the latest emergency threatening Gotham City. Racing the the scene of the crime in the Batmobile, Batman and Robin must (with the help of their trusty Bat-utility-belt) thwart the efforts of a variety of master criminals, including The Riddler, The Joker, Catwoman, and The Penguin.