My Little Pony Equestria Girls is an animated feature film starring characters resembling the main cast of My Little Pony Friendship is Magic as teenage girls. Princess Twilight Sparkle's crown, which is the element of magic, is stolen by Sunset Shimmer who disappears into a mirror that leads into another world, the human world. Without the crown, all the other Elements of Harmony will have no power to protect Equestria, so Twilight and Spike go through the mirror to chase after the culprit to retrieve her crown. They discover themselves in a new world where Twilight is a teenage girl and Spike is a talking dog, and find friends that resemble their friends from Equestria. Twilight works with her friends to become Princess of the Fall Formal to win her crown back from the human Sunset Shimmer and to 'change the destiny of these two parallel worlds'.

A magical empire has suddenly appeared in the arctic north of Equestria, and Princess Celestia needs Twilight Sparkle and her friends to find a way to protect it. While her friends try to keep the Crystal Ponies occupied at the Crystal Fair, Twilight Sparkle searches for the hidden Crystal Heart that is the key to keeping their empire safe from harm.

A magical empire has suddenly appeared in the arctic north of Equestria, and Princess Celestia needs Twilight Sparkle and her friends to find a way to protect it. While her friends try to keep the Crystal Ponies occupied at the Crystal Fair, Twilight Sparkle searches for the hidden Crystal Heart that is the key to keeping their empire safe from harm.

Overwhelmed with having to choose just one friend to spend time with, Pinkie Pie uses a magic pool to clone herself.

The Cutie Mark Crusaders are crushed when Babs Seed rejects their invitation to become a fellow Crusader and instead become a bully.

When Trixie returns to Ponyville, she defeats Twilight and banishes her from town.

Over a campfire, Rainbow Dash decides to share some imaginative and spooky tales, causing Scootaloo to have frightening, vivid nightmares; Scootaloo wants to get rid of her nightmares but doesn't want the others to think she is spineless.

Rainbow Dash receives an invitation to attend the Wonderbolt Academy but questions whether she is Wonderbolt material.

Granny Smith agrees to let Applejack lead the Apple Family reunion but Applejack loses sight of what is truly important.

After Applejack saved Spike, he insists on fulfilling the Nobel Dragon Code by repaying her with excessive help around the farm.

Princess Celestia tasks the ponies with reforming Discord, a mischievous spirit of disharmony, but Fluttershy is the only one who is willing to give him a chance.

Spike finds himself in over his head after convincing Twilight and her friends to let him pet-sit all of their critters in exchange for a few gems.

Twilight and her friends, invited by Princess Cadance, welcome the inspector for the Equestria Games.

Twilight Sparkle accidentally casts a spell that switches the Cutie Marks and destinies of all her friends, and the only way she can reverse the spell is by writing her own magic. Little does she know that by achieving this great accomplishment, she will fulfill her destiny and emerge a princess.